← Nutrients · Metabolic Health

Vitamin C

Vitamin Foundational Antioxidant Cofactor

Holds collagen together, recycles other antioxidants, and powers your stress response.

At a glance

Essential
Strong human evidenceSome human evidence

How to take it

  • 75–90 mg a day (+35 mg if you smoke)
  • Black currants, red peppers, kiwi, broccoli, strawberries, oranges
  • Absorption depends on sodium (see Sodium)

Watch out

  • Upper limit 2,000 mg a day

Signs you're low

  • Bleeding gums
  • Slow wound healing
  • Fatigue
The Research

Mechanisms, studies and evidence grades for each claim above.

Intake

RDA ~75 mg/day (women), 90 mg/day (men); smokers +35 mg/day. Upper limit 2,000 mg/day.3 Humans lost the capacity to synthesize vitamin C and must obtain it entirely from diet.3

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Transport and absorption

Absorbed via sodium-dependent transporters — SVCT1 in the gut, SVCT2 in peripheral tissue and brain. Transport is coupled to sodium gradients, which is the mechanistic link back to electrolyte status.7

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Collagen synthesis (key mechanism)

Maintains iron in its reduced form (Fe²⁺), which enables prolyl and lysyl hydroxylase activity — the step that stabilizes the collagen triple helix. Deficiency produces impaired collagen stability, which is the mechanism behind scurvy.1 (Strong — human evidence) The same mechanism underlies wound healing: systematic review of clinical trials finds supplementation improves healing outcomes in several tissue-injury contexts, though study quality is mixed.16 (Moderate — mixed trial quality)

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Systems

Oxidative stress. Acts as a reducing agent that neutralizes reactive oxygen species; protects lipids, proteins, and DNA from oxidative damage; regenerates other antioxidants including vitamin E.2 (Strong)

Brain. Concentrated in neurons; supports antioxidant defense2 and neurotransmitter synthesis, including norepinephrine;5 transported in via SVCT2.7

Immune. Supports leukocyte function and reduces oxidative stress during infection.6

Heart. Maintains tetrahydrobiopterin (BH4), a cofactor for nitric oxide synthase — supports vascular nitric oxide production and, per the glycine/collagen entry, may help reduce atherosclerotic plaque via this route.89 (Moderate)

Gut. Enhances non-heme iron absorption by reducing Fe³⁺ to Fe²⁺;4 absorbed itself via SVCT1 in the intestinal epithelium.7

Adrenal / stress response. The adrenal cortex and medulla hold some of the highest vitamin C concentrations of any tissue; ACTH stimulation triggers measurable vitamin C secretion from the gland into circulation as part of the stress response, and ascorbate is a cofactor for dopamine β-hydroxylase, the enzyme that produces the stress hormone norepinephrine.115 (Moderate — human evidence)

Reproductive health. Concentrated in the corpus luteum; supplementation raised serum progesterone and pregnancy rate in women with a luteal phase defect.12 In men, it makes up the majority of seminal fluid's antioxidant capacity, and small trials found supplementation improved sperm count, motility, and morphology.13 (Moderate — small human trials)

Bone. Higher dietary intake correlates with greater bone mineral density in observational cohorts, consistent with its cofactor role in synthesizing the type I collagen that forms bone's organic matrix.14 (Moderate — observational)

Histamine metabolism. Experimental depletion-repletion studies show blood histamine rises as vitamin C status falls, consistent with a cofactor role in histamine breakdown via diamine oxidase.10 (Moderate — human depletion/repletion study)

Cellular energy. Ascorbate is classically described as a cofactor for the two hydroxylases in carnitine biosynthesis — the pathway that shuttles fatty acids into mitochondria for beta-oxidation — and human depletion studies show plasma carnitine falls alongside vitamin C.10 However, a knockout-mouse study found vitamin C is not strictly essential for carnitine synthesis in vivo.15 (Weak — contested)

Detoxification. As a reducing agent, vitamin C plausibly helps quench reactive intermediates generated during phase I cytochrome P450 liver metabolism, but this is inferred from its general antioxidant mechanism2 rather than demonstrated in direct outcome trials on detox pathways. (Weak — mechanistic only)

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Deficiency effects

Scurvy: impaired collagen synthesis presenting as bleeding gums, poor wound healing, and fatigue.1

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Cross-reference: skin / connective tissue

Vitamin C is a documented, high-confidence cofactor for prolyl and lysyl hydroxylase in collagen biosynthesis.1 (Strong — human evidence) See the Glycine / Collagen entry for the full synthesis chain this feeds into.

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Top foods

FoodVitamin C (approx.)
Black currants~200 mg / cup (~222% DV)
Red bell pepper~190 mg / cup raw (~211% DV)
Kiwi~130–170 mg / 2 fruit (~145–189% DV)
Brussels sprouts~95 mg / cup cooked (~106% DV)
Broccoli~80–100 mg / cup cooked (~89–111% DV)
Strawberries~85 mg / cup (~94% DV)
Orange~70–80 mg / medium fruit (~78–89% DV)
Orange juice~60–90 mg / 8 oz (~67–100% DV)
Tomato~17 mg / medium fruit (~19% DV)
Potato (baked)~20 mg / medium (~22% DV)
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References

  1. Pinnell, 1985 — vitamin C regulates collagen biosynthesis. PMID 3008449
  2. Glutathione metabolism and functions (review). 2004. PMID 15245925
  3. Vitamin C requirements (review). 2012. PMID 22806044
  4. Vitamin C enhances iron absorption. PMID 6940487
  5. Vitamin C & norepinephrine synthesis. PMID 6139316
  6. Vitamin C & immune function (review). 2013. PMID 23440782
  7. Takanaga et al., 2004 — SVCT sodium-dependent vitamin C transport. PMID 12845532
  8. Nitric oxide / BH4. PMID 10357326
  9. Nitric oxide / BH4. PMID 15178568
  10. Johnston, 1996 — vitamin C depletion alters blood histamine and plasma free carnitine. PMID 8951736
  11. Padayatty et al., 2007 — human adrenal glands secrete vitamin C in response to ACTH. PMID 17616774
  12. Henmi et al., 2003 — ascorbic acid and serum progesterone in luteal phase defect. PMID 12909517
  13. Akmal et al., 2006 — vitamin C and human semen quality. PMID 17004914
  14. Hall et al., 1998 — dietary vitamin C intake and bone mineral density (PEPI study). PMID 9701620
  15. Furusawa et al., 2008 — vitamin C not essential for carnitine biosynthesis in vivo (knockout mice). PMID 18758058
  16. Bechara et al., 2022 — systematic review on the role of vitamin C in tissue healing. PMID 36009324